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Project 1: Cold Atmospheric Pressure Plasma Against Drug Resistant Microorganisms for Wound Healing


            In physics, plasma refers to particles or charged particles of matter, or the state of charged gas. When matter is heated or subjected to electric fields, it stimulates the breaking up which generates charged particles and radicals, ultraviolet radiation (UV) and heat. At present, there has been wide interest of using cold atmospheric pressure plasma in the field of biomedical sciences and hygiene as can be seen from the system / device production produced by plasma technology for using with humans directly, such as plasma skin regeneration, which has been certified by the Food and Drug Administration in the US and Europe since 2005 and 2006 respectively, focusing on research and development of plasma system / device at room temperature to cover medical, dentistry and hygiene applications.


           Cold atmospheric pressure plasma system will produce plasma particles containing electrons, positive / negative ions and radicals, such as O, OH, NO or others based on carrier gas and mixed gases compositions. The determination of particle types depends on the objectives of biomedical science applications. For example, the applications of cold atmospheric pressure plasma for the treatment of infected and chronic wounds due to the need for a new effective treatment in eliminating antibiotic-resistant bacteria, but at the same time, does not hurt good tissues. In turn, it helps to stimulate the formation of new blood vessels by using a simple, safe, and economical method.

Figure 1.1 Cold atmospheric pressure plasma for the treatment of infected wounds.

            This research project aims to develop a cold atmospheric pressure plasma system for the treatment of drug-resistant microorganisms for wound healing in the vitro biofilm by using the electrical power of cold plasma not over 2-3 watts per square centimeter. This is a safe power for wound tissue application, but it has the potential to eliminate bacteria, as well as to stimulate the outer epidermis. In physics, plasma particles, UV power, electrical power, and safety standards of cold atmospheric pressure plasma system will be analyzed. In biochemistry and biomolecules, transfer process, interaction of OH and NO radicals of cold atmospheric pressure plasma in removing the biofilm of drug-resistant bacteria, such as Methicillin-resistant Staphylococcus aureus (MRSA) and Enterococcus faecalis, by studying the OH particle elimination of bacterial cells in the biofilm including the proliferation of skin cells from the activation mechanism of NO radicals, will be examined.


Principal Investigator: Associate Professor Dr. Dheerawan Boonyawan 1)
Collaborators: Dr. Nirut Pussadee 1), Dr. Sureeporn Sarapirom 2), Dr. Kewalin Inthanon 3), Prof. Kittipan Rerkasem, M.D. 4), Clin. Prof. Apirag Chuangsuwanich, M.D. 5), Dr. Apiwat Wichaikhum 1)
Affiliated Institutions: 1) Department of Physics and Materials Science, Faculty of Science, Chiang Mai University, 2) Faculty of Science, Maejo University, 3) Faculty of Science and Technology, Thammasat University, 4) Faculty of Medicine, Chiang Mai University, 5) Faculty of Medicine, Mahidol University